Gẹgẹbi ọna asopọ ti amino acid ati suga, peptide suga le pin si awọn ẹka mẹrin: O glycosylation, C a N glycosylation, saccharification ìri ati asopọ GPI (glycophosphatidlyinositol).
1. N-glycosylation glycopeptides ti wa ni kq ti N-acetamide glukosi ni idinku opin ti awọn glycan pq (Glc-Nac) ti sopọ si N atom lori ẹgbẹ amide ti awọn ẹgbẹ pq ti diẹ ninu awọn Asn ni peptide pq, ati awọn Asn. ti o lagbara lati sopọ pq glycan gbọdọ wa ni AsN-X-Ser / Thr (X! = P) ni ero ti o ṣẹda nipasẹ awọn iṣẹku.Awọn suga jẹ N-acetylglucosamine.
N-glycosylation ti a ṣe atunṣe glycopeptide igbekale
2. Ilana ti O-glycosylation jẹ rọrun ju N-glycosylation.Glycopeptide yii kuru ni gbogbogbo ju glycan, ṣugbọn o ni awọn oriṣi diẹ sii ju N-glycosylation.Ser ati Thr ni gbogbogbo le jẹ glycosylated ninu pq peptide.Ni afikun, awọn glycopeptides wa ti a ṣe ọṣọ pẹlu tyrosine, hydroxyl, ati hydroxyproline glycosylation.Ipo ọna asopọ jẹ atomu atẹgun hydroxyl lori ẹwọn ẹgbẹ ti iyokù.Awọn suga ti o ni asopọ jẹ galactose tabi N-acetylgalactosamine (Gal&GalNAc) tabi glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Eniyan/ManNAc), ati bẹbẹ lọ.
O-glycosylation ṣe atunṣe eto naa
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
N-acetylglucosamine kan (GlcNAc) glycosylation kan so awọn ọlọjẹ O-GlcNAc pọ mọ atomu oxygen hydroxyl ti serine tabi aloku threonine ti amuaradagba kan.O-GlcNA glycosylation jẹ ohun ọṣọ monosaccharide O-GlcNAc laisi itẹsiwaju glycan;Bii peptide phosphorylation, O-GlcNAc glycosylation ti glycopeptides tun jẹ ilana ọṣọ amuaradagba ti o ni agbara.Ohun ọṣọ O-GlcNAc ajeji le fa ọpọlọpọ awọn arun bii àtọgbẹ, awọn arun inu ọkan ati ẹjẹ, awọn èèmọ, Arun Alzheimer ati bẹbẹ lọ.
Awọn aaye Glycosylation ti glycopeptides
Awọn ẹya ipilẹ ti polypeptide ati awọn ẹwọn suga ni asopọ si awọn ẹwọn amuaradagba nipasẹ awọn iwe adehun covalent, ati awọn aaye ti o so awọn ẹwọn suga ni a pe ni awọn aaye glycosylation.Niwọn igba ti ko si awoṣe lati tẹle biosynthesis ti awọn ẹwọn suga glycopeptide, awọn ẹwọn suga oriṣiriṣi yoo so mọ aaye glycosylation kanna, ti o yori si eyiti a pe ni inhomogeneity microscopic.
Glycosylation ti glycopetides
1. Ipa ti glycopeptide glycosylation lori itọju ailera-ipa ti awọn ọlọjẹ iwosan
Ninu ọran ti awọn ọlọjẹ-itọju ailera, glycosylation tun ni ipa lori idaji-aye ati ifọkansi ti awọn oogun amuaradagba ni vivo
2. Soluble glycopeptide glycosylation ati awọn ọlọjẹ
Awọn ijinlẹ ti fihan pe awọn ẹwọn suga lori dada ti awọn ọlọjẹ le mu ilọsiwaju molikula ti awọn ọlọjẹ dara
3. Glycopeptide glycosylation ati imunogenicity amuaradagba
Ni ọwọ kan, awọn ẹwọn suga lori dada ti awọn ọlọjẹ le fa awọn idahun ajẹsara kan pato.Ni ida keji, awọn ẹwọn suga le bo awọn aaye kan lori aaye amuaradagba ati dinku ajẹsara rẹ
4. Glycopeptide glycosylation ti o mu iduroṣinṣin amuaradagba pọ si
Glycosylation le ṣe alekun iduroṣinṣin ti awọn ọlọjẹ si ọpọlọpọ awọn ipo denaturation (gẹgẹbi awọn denaturant, ooru, ati bẹbẹ lọ) ati yago fun akojọpọ awọn ọlọjẹ.Ni akoko kanna, awọn ẹwọn suga lori dada ti awọn ọlọjẹ tun le bo diẹ ninu awọn aaye ibajẹ proteolytic ti awọn ohun elo amuaradagba, nitorinaa jijẹ resistance ti awọn ọlọjẹ si awọn ọlọjẹ.
5. Glycopeptide glycosylation ti o ni ipa lori iṣẹ ṣiṣe ti ibi ti awọn ohun elo amuaradagba
Yiyipada glycosylation amuaradagba le tun jẹ ki awọn ohun elo amuaradagba ṣe agbekalẹ awọn iṣẹ iṣe ti ibi tuntun
Akoko ifiweranṣẹ: Oṣu Kẹjọ-03-2023