Awọn peptides ti a ṣe atunṣe-methylation, ti a tun mọ ni awọn peptides ti a mọ ni methylation, jẹ awọn ohun ọṣọ post-translational (PTMs) amuaradagba ati ṣe ipa ilana bọtini ni fere gbogbo awọn iṣẹ igbesi aye ninu awọn sẹẹli.Awọn ọlọjẹ jẹ catalyzed nipasẹ methyltransferase lati gbe awọn ẹgbẹ hydroxyl lọ si awọn iṣẹku amino acid kan pato fun isomọ covalent.Methylation jẹ ilana iyipada ti o le yipada nipasẹ awọn demethylases.
Awọn ijinlẹ ti fihan pe awọn amino acid methylated / demethylated ti o wọpọ nigbagbogbo jẹ lysine (Lys) ati arginine (Arg).Awọn ijinlẹ ti rii pe histone lysine methylation ni ọpọlọpọ awọn iṣẹ iṣe ti ara bii itọju sẹẹli ati pipin, X chromosome inactivation, ilana transcription ati idahun ibajẹ DNA.", nigbagbogbo ni ipa lori ifunmi chromatin ati ki o ṣe atunṣe ikosile jiini."Histone arginine methylation ṣe ipa pataki ninu ilana ti transcription pupọ ati pe o le ni ipa lori ọpọlọpọ awọn ilana ẹkọ nipa ẹkọ nipa ẹkọ nipa ti ara ninu awọn sẹẹli, pẹlu atunṣe DNA, gbigbe ifihan agbara, idagbasoke sẹẹli, ati carcinogenesis.Nitorinaa, Guopeptide Biology ti ni idagbasoke pataki imọ-ẹrọ ti awọn peptides ohun ọṣọ methyl, eyiti o jẹ iyipada nipasẹ awọn onimọ-jinlẹ lẹhin itumọ amuaradagba (PTMS) lati ṣe iranlọwọ ninu iwadii naa.
Iyipada Methylation (Me1, Me2, Me3)
Fmoc-Lys to gaju (Me, Boc) -OH, Fmoc-Lys (Me2) -OH, Fmoc-Lys (Me3) -OH.HCL, Fmoc-Arg (Me, Pbf) -OH, Fmoc-Arg (Me) 2-OH.HCl (asymmetrical), F ni a lo moc-Arg (me) 2-OH.HCl (symmetrical) ati awọn ohun elo aise miiran ti a ṣe nipasẹ FMOC ilana iṣelọpọ ti o lagbara lati gba Lys ati Arg methylated peptides, ati awọn ọja naa. won wẹ nipa HPLC.Awọn iwoye ibi-pupọ ti o yẹ, awọn chromatograms HPLC ati COA ti pese fun ọja ti o pari.
Akoko ifiweranṣẹ: Oṣu kọkanla-24-2023